Spectrofluorimetric assay of amlodipine and perindopril in their raw materials, pharmaceutical formulations and spiked human plasma through the formation of complexes with Eosin Y

Abstract For quantitation of amlodipine (AML) and perindopril (PER) in their authentic, pharmaceutical formulations spiked human plasma, a simple, sensitive, validated inexpensive spectrofluorimetric method has been developed. The proposed is developed to be based on quantitative quenching effect two antihypertensive drugs Eosin Y's native fluorescence which was achieved by developing binary complexes between each the cited an acidic environment using acetate buffer (pH 4.4) with Y. Fluorescence recorded at 544 nm after excitation 425 nm. AML PER, calibration curves were obtained over range 0.3–3.0 µg/mL 0.2–2.0 µg/mL, respectively, correlation coefficients 0.9993 0.9995, respectively. according ICH guidelines. regarded new sensitive. As result, might used estimate quality biological fluid.